Plate-based selection allows even more control over the transfer step, with visual identification of colonies.Įither obviously larger or otherwise morphologically desired (e.g., Kuthan et al., 2003 ) candidates can be serially inoculated to fresh plates, or, on the other end of the spectrum, as little selection as possible can be imposed by selecting random colonies. The relative amount of time cells spend in each phase of growth may change over the course of one of these experiments, particularly as lag phase shortens and maximal growth rate improves. Here, one nutrient is lowered to the point that it uniquely runs out first and limits the saturated biomass of the culture. Nutrient exhaustion is a popular selection scheme for batch transfer experiments, brought to prominence by experiments in bacteria by Lenski and colleagues (reviewed in Elena and Lenski, 2003 ) and adapted for yeast by Zeyl (2005).
Importance of serial dilution in research full#
In these cases, full growth curves may not be desired, as improved performance with respect to nutrient exhaustion or stationary phase may be separate outcomes unrelated to the main selection applied by the experimenter. The easiest selections to understand are improvements to growth when maximal performance is attenuated either by exogenous or genetic means.
Selection pressures of a number of types can be accommodated. Serial dilution has many advantages: the materials necessary are typically already present in the lab and require no special engineering.Ĭonditions can be adjusted as the experiment progresses (e.g., drug concentrations increased as drug resistance improves). View chapter Purchase book Read full chapter URL: Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis Maitreya J.ĭunham, in Methods in Enzymology, 2010 3.1 Serial dilution Serial dilution generally refers to selection preformed in the standard growth regimes typically used in the lab: flasks, test tubes, solid media, or 96-well plates.Ĭultures are usually allowed to grow through a normal growth curve, with daily transfer of a small volume of the expanded culture into fresh medium. Advantages Of Serial Dilution Full Chapter URL
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Advantages Of Serial Dilution Download As PDFĪlternatively, the dilutions can be prepared in 96-well plates and 0.1 mL transferred to the Lab-Tek slides.įigure 17.3.
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